A Short Method for the Purification of Arginase from Ox Liver.

L( + )-Arginine hydrochloride was obtained from the Sigma Chemical Co., St Louis, Mo., U.S.A. The Arlco jackbean meal used in the preparation of urease was supplied by Hopkin and Williams Ltd., Chadwell Heath, Essex. The 'manganese-maleate solution' used in the preparation of arginase was made by dissolving 8-9 g. of MnSO4,4H20 and 1-8 g. of disodium maleate in glassdistilled water and making the volume up to 1 1. Buffers. 0-1 M-Glycine buffer was prepared by dissolving 7-5 g. of glycine in 200 ml. of water, adjusting the pH of the solution to 9 0 with 0-1N-NaOH and making the volume up to 11. with glass-distilled water. 3M-Acetate buffer, pH 4-6, was prepared by dissolving 408 g. of hydrated sodium acetate in 500 ml. of water, adding 170 ml. of acetic acid and making the volume up to 1 1. with glass-distilled water.